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4876

p-Nitrophenyl Phosphate, disodium salt, hexahydrate

≥99% (HPLC), crystalline solid, alkaline phosphatase-based ELISA assay substrate, Calbiochem®

Synonym(s):

p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, pNPP, 2Na

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Size/SKUAvailabilityPrice
1 g
Please contact Customer Service for Availability
1 180,00 CZK
5 g
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5 780,00 CZK

About This Item

Empirical Formula (Hill Notation):
C6H4NO6P · 2Na · 6H2O
CAS Number:
Molecular Weight:
371.14
UNSPSC Code:
41116158
NACRES:
NA.77
MDL number:
Assay:
≥99% (HPLC)
Form:
crystalline solid
Storage condition:
OK to freeze, protect from light

1 180,00 CZK


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Product Name

p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, p-Nitrophenyl Phosphate, Disodium Salt, Hexahydrate, CAS 4264-83-9, is an excellent substrate for alkaline phosphatase-based ELISA assays. Produces yellow soluble end product (405-410 nM).

Quality Segment

assay

≥99% (HPLC)

form

crystalline solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, protect from light

impurities

≤0.005% p-nitrophenol (weight), ≤1% free phosphate (weight)

color

light yellow

solubility

water: 5 mg/mL

shipped in

ambient

storage temp.

2-8°C

SMILES string

[Na+].[Na+].[P](=O)([O-])([O-])Oc1ccc(cc1)[N+](=O)[O-]

InChI

1S/C6H6NO6P.2Na/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12;;/h1-4H,(H2,10,11,12);;/q;2*+1/p-2

InChI key

VIYFPAMJCJLZKD-UHFFFAOYSA-L

General description

Excellent substrate for alkaline phosphatase-based ELISA assays. Produces a yellow soluble end product that can be read at 405 nm to 410 nm. Both kinetic and endpoint determinations can be performed. ε max: ≥9600 cm-1 M-1 at 311 nm in 0.4 M Na2CO3.

Biochem/physiol Actions

Cell permeable: no
Primary Target
Substrate for alkaline phosphatase-based ELISA assays
Product does not compete with ATP.
Reversible: no

Preparation Note

Unstable in solution; reconstitute just prior to use.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)


Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable



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Questions

  1. Saya membutuhkan bahan p-Nitrophenyl Phosphate disodium salt hexahydrate untuk penilitian laboratorium sekolah. Total kebutuhan experimen kami hanya sekitar 0.1-0.2 gram. Apakah memungkinkan untuk pemesanan kuantitas khusus di bawah 1 gram?

    1 answer
    1. Unfortunately, the smallest quantity of this product is the 1 gram package size. However, product N9389 a 5 mg tablet of pNPP disodium salt hexahydrate. The smallest package size is a 50 tablets for a total of 250mg. Please see the link below to review this product:
      https://www.sigmaaldrich.com/product/sigma/n9389

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