AB3085

Anti-Na+/H+ Exchanger-3 Antibody

Chemicon^®, from rabbit

• Synonym(s): NHE3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA, WB
• Citations: 16

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable, western blot: suitable
• NCBI accession no.: NP_004165
• UniProt accession no.: P48764
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: rat ... Slc9A3(24784)

Description

General description

93 kDa

Immunogen

A 22 AA peptide within the cytoplasmic, c-terminal domain of the rat NHE3 (Brant et al. 1995; Orlowsji et al. 1992; Chris Yun et al. 1995; Josette & Pouyssegur 1995). Control peptide is available, cat# AG779.

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Na+/H+ Exchanger-3 Antibody is validated for use in ELISA, WB for the detection of Na+/H+ Exchanger-3.

Western blot: 1-10 μg/mL using Chemiluminescence technique. A band of approximately 90 kD has been detected in brush border membrane (Yohsioka et al. 1997). ELISA: 1:100,000 using 50-100 ng control peptide/well.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Recognizes NHE3.

The immunogen sequence shares 90% homology with mouse NHE3.

Physical form

Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA.

ImmunoAffinity Purified

Preparation Note

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Kidney, jejunum, colon, and stomach tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2