AB6000

Anti-TIMP-3 Antibody, CT

from rabbit, purified by affinity chromatography

• Synonym(s): MIG-5, TIMP metallopeptidase inhibitor 3, Tissue inhibitor of metalloproteinases 3
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 13

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: horse, human, rat
• species reactivity (predicted by homology): pig (based on 100% sequence homology), primate (based on 100% sequence homology), equine (based on 100% sequence homology), canine (based on 100% sequence homology), monkey (based on 100% sequence homology), mouse (95% homology), bovine (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_000353
• UniProt accession no.: P35625
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... TIMP3(7078)

Description

General description

Bands may be observed for reduced protein bands at ~ 24 kDa (unglycosylated) and ~ 30 kDa (glycosylated). Additional TIMP-3 bands MAY be visible at 50 kDa (dimer), 12 kDa, and 15 kDa (breakdown products) and sample dependent.

Tissue inhibitor of metalloproteinases 3 (TIMP-3) is a member of the TIMP family. The TIMPs are so named because they are slow, tight binding endogenous inhibitors of the Matrix Metalloproteinases (MMPs). The four TIMPs have different inhibition constants for the different MMPs studied. TIMPs have been shown to have activity against the ADAMs family of proteinases. TIMP-3 is an efficient "sheddase" inhibitor, inhibiting ADAM-17 (TACE) at the low nanomolar levels seen with MMP inhibition. The other ADAMs proteinases have not yet been assayed for TIMP inhibition, but TIMP-2 seems to be much less active on ADAM-17 than isTIMP-3. TIMP-3 is thought to be constitutively produced by many cell types and is inducible in others. The TIMP-3 localization differs from the other 3 TIMPs, and is thought to be primarily deposited into the extracellular matrix.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to human TIMP-3 at the C-terminus.

Application

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected TIMP-3 in C6 cells.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

This Anti-TIMP-3 Antibody, C-terminus is validated for use in WB, IC for the detection of TIMP-3.

Biochem/physiol Actions

The antibody recognizes TIMP-3 at the C-terminus. Does not appear to cross react with TIMP-1 or TIMP-2.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HL-60 cell lysate in PMA-conditioned media.

Evaluated by Western Blot using an HL-60 cell lysate supplemented with PMA-conditioned media.

Western Blot Analysis: 0.5 µg/ml of this antibody detected TIMP-3 on 10 µg of HL-60 in PMA-conditioned media.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: AB802

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1