ABC10

Anti-Megf10 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Multiple epidermal growth factor-like domains protein 10, Multiple EGF-like domains protein 10
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702

Properties

• Product Name: Anti-Megf10 Antibody, from rabbit, purified by affinity chromatography
• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse
• technique(s): immunofluorescence: suitable; western blot: suitable
• NCBI accession no.: NP_001001979
• UniProt accession no.: Q6DIB5
• shipped in: wet ice
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: mouse ... Megf10(70417)

Description

Analysis Note

Control
Mouse retina tissue lysate

Evaluated by Western Blotting in Mouse Brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Megf10 in Mouse Brain tissue lysate.

Application

Detect the Megf10 protein using this Anti-Megf10 validated for use in WB & IF.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Immunofluorescence Analysis: A representative lot was used to detect Megf10 in P7 caudal brain tissue and retina cells by an independent laboratory. (Image courtesy form the laboratory of Dr. Joshua Sanes, Harvard University).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Biochem/physiol Actions

Other homologies: Rat (98% sequence homology) and Human (92% sequence homology).

This antibody recognizes Megf10 at the cytoplasmic domain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Multiple epidermal growth factor-like domains protein 10 (Megf10) is a type 1 transmembrane protein with multiple EGF-like extracellular domains. Megf10 is highly expressed in the brain, and plays an important role in phacocytosis of apoptotic cells. This role may be performed through the interaction of Megf10 with the ATP-binding cassette transporter (ABCA1) protein. Previous studies have suggested that Megf10 may be involved in the uptake of amyloid β peptides in the brain. Megf10 may therefore play a role Alzheimer’s disease.

~130 kDa observed

Immunogen

Epitope: Cytoplasmic domain

Histidine-tagged recombinant protein corresponding to the cytoplasmic domain of mouse Megf10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable