ABE40

Anti-PABPC1/C3 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): Polyadenylate-binding protein 1, PABP-1, Poly(A)-binding protein 1
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ICC, WB
• Citations: 1

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human
• species reactivity (predicted by homology): canine (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, western blot: suitable
• NCBI accession no.: NP_002559
• UniProt accession no.: P11940
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PABPC1(26986)

Description

General description

Poly(A)-binding proteins, cytoplasmic 1 (PABPC1), and cytoplasmic 3 (PABPC3) are RNA-binding proteins with important roles in post-translational control and metabolism of mRNA. Although both proteins bind to the poly(A) tail of mRNA, PABPC3 binds with a lower affinity than PABPC1. Studies have associated these proteins with tumorigenesis. Decreased expression of PABPC1 in esophageal cancer seems to associate with tumor development and poor prognosis after surgery. PABPC1 can shuttle through the nuclear membrane and is thought to associate with newly synthesized poly(A) transcripts. PABPC1 has also been linked to microRNA mediated silencing when in contact with GW182. PABPC3 is thought to be specific to human testis and controls RNA stability.

~71 kDa observed

Immunogen

KLH-conjugated linear peptide corresponding to human PABPC1/C3. The immunogen sequence used is 100% identical to both PABPC1 and PABPC3.

Application

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected PABPC1/C3 in NIH/3T3 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
RNA Metabolism & Binding Proteins

Use Anti-PABPC1/C3 (Rabbit Polyclonal Antibody) validated in ICC, WB to detect PABPC1/C3 also known as Polyadenylate-binding protein 1, PABP-1.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PABPC1/C3 on 10 µg of HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1