CB1024

Anti-ACTN4 Mouse mAb (4D10)

liquid, clone 4D10, Calbiochem^®

• Synonym(s): Anti-Actinin α4
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 4D10, monoclonal
• Species reactivity: mouse, human, rat
• Application: —
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4D10, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: mouse, human, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (ELISA (see coments); Immunoblotting (1-5 µg/mL); Immunocytochemistry (20 µg/mL); Paraffin Sections (3 µg/mL))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ACTN4(81)

Description

General description

Anti-ACTN4, mouse monoclonal, clone 4D10, recognizes the ~90-100 kDa ACTN4 in PC-12, HeLa, Raw 264.7, MCF-7, NIH3T3 cells & in human testes. It is validated for ELISA, WB, ICC, and paraffin sections.

Purified mouse monoclonal antibody. Recognizes the ~90-100 kDa ACTN4 protein.

Recognizes the ~90-100 kDa ACTN4 protein in PC-12, HeLa, Raw 264.7, MCF-7, and NIH3T3 cells and human testes tissue.

Immunogen

A recombinant polypeptide corresponding to amino acids 592-702 of human ACTN4, espressed as a GST fusion protein

Application

ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (20 g/ml)
Paraffin Sections (3 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells

Positive Control
PC-12 cells, HeLa cells, Raw 264.7 cells, MCF-7 cells, NIH3T3 cells, Human testes tissue

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

Yamada, S., et al. 2010. Int. J. Oral Maxillofac. Surg.39, 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: nwg