CC115

Chicken Tenascin

from embryonic chicken brains, liquid, 0.755 mg/mL, suitable for cell culture

• Synonym(s): Cytotactin, Tenascin-C
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.75
• Biological source: chicken
• Form: liquid
• Technique(s): cell culture | mammalian: suitable
• Concentration: 0.755 mg/mL
• Mol wt: large molecular weight polypeptide 190 kDa, large molecular weight polypeptide 200 kDa, large molecular weight polypeptide 220 kDa

Properties

• Product Name: Chicken Tenascin,
• biological source: chicken
• Quality Segment: 100
• form: liquid
• mol wt: large molecular weight polypeptide 190 kDa, large molecular weight polypeptide 200 kDa, large molecular weight polypeptide 220 kDa
• manufacturer/tradename: Chemicon^®
• concentration: 0.755 mg/mL
• technique(s): cell culture | mammalian: suitable
• input: sample type hematopoietic stem cell(s); sample type mesenchymal stem cell(s); sample type pancreatic stem cell(s); sample type induced pluripotent stem cell(s); sample type neural stem cell(s); sample type epithelial cells; sample type: mouse embryonic stem cell(s)
• UniProt accession no.: Q90W79
• shipped in: dry ice
• storage temp.: −20°C
• Gene Information: chicken ... CNTN5(395317)

Description

General description

Tenascin is a large glycoprotein that forms disulfide-linked hexamers. It is made up of repeated structural domains including EGF-like repeats, fibronectin type III repeats, and a region homologous to fibrinogen. Splice variants of tenascin differ in their number of fibronectin type III repeats. Tenascin is expressed in distinctive spatial and temporal patterns in most embryonic tissues at sites of tissue remodeling and cell migration. There is little expression of tenascin in adult tissues except, again, at sites of tissue remodeling and cell migration such as surrounding tumors, in healing wounds, and in branching or damaged blood vessels. Tenascin binds to several cell-surface receptors and mediates cell-substrate adhesion. Nevertheless, tenascin is frequently referred to as an anti-adhesion molecule because for a variety of cell types the presence of tenascin inhibits adhesion to other ECM proteins present. This effect occurs because the binding of tenascin to certain receptors inhibits cell spreading and thereby blocks the stabilization of adhesion that results from cell preading in vitro assays.

Physical form

Provided as a liquid in PBS, containing no preservatives.

Preparation Note

Store at -20°C for up to 12 months. Avoid repeated freeze/thaw.

Analysis Note

Isolated from embryonic chicken brains by chromatographic methods. Contains three characteristic polypeptide chains (220, 200, and 190 kDa) that migrate on SDS gels as a high molecular weight multimer under non-reducing conditions.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2