GF321

Fibroblast Growth Factor-Acidic human

animal free, recombinant, expressed in E. coli, suitable for cell culture

• Synonym(s): FGF-1, alpha FGF, FGFa
• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.77
• Impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)

Properties

• Product Name: FGF-acidic, Human Recombinant ; Animal Free,
• impurities: <0.1 μg/mg protein endotoxin (<1 EU/μg)
• Quality Segment: 100
• UniProt accession no.: P05230
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

FGF-acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGFacidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF-acidic has the ability to signal through all the FGF receptors. Recombinant human FGFacidic is a 16.0 kDa protein consisting of 141 amino acid residues.

Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.

Application

Research Category
Stem Cell Research

Research Sub Category
Growth Factors & Receptors

Physical form

Product is presented in 5 mM Sodium Phosphate, pH 8.0 + 100mM NaCl. and is filtered through a 0.2 micron filter before lyophilization.

Preparation Note

Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in 5 mM Sodium Phosphate, pH 8.0 to a concentration of <0.5 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Analysis Note

Bioactivity assay:
Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ≤ 10 ng/ml corresponding to a specific activity of ≥ 1 x 10^5 units/mg.
Assay #2: The ED50 was determined by a cell proliferation
assay using balb/c 3T3 cells is ≤ 0.5 ng/ml in the presence
of 10 μg/ml heparin, corresponding to a specific activity of
≥ 2 x 10^6 units/mg.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• WGK: WGK 3