LP02

Anti-LDL Receptor Mouse mAb (15C8)

liquid, clone 15C8, Calbiochem^®

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 15C8, monoclonal
• Species reactivity: human, bovine
• Application: —
• Citations: 1

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 15C8, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, bovine
• should not react with: rabbit
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (ELISA (0.5-1 µg/mL); Flow Cytometry ; Frozen Sections (1-5 µg/mL); Immunoblotting (1-5 µg/mL, ; Immunocytochemistry (1-5 µg/mL, IF); Immunoprecipitation (1-2 µg/sample))
• isotype: IgG2a
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LDLR(3949)

Description

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with Sp2/0-Ag14 myeloma cells (see application references). Recognizes the ~160 kDa LDL Receptor protein.

Recognizes the ~160 kDa LDL receptor protein in HT1080 and HepG2 cells.

This Anti-LDL Receptor Mouse mAb (15C8) is validated for use in ELISA, Flow Cytometry, Frozen Sections, Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of LDL Receptor.

Immunogen

Bovine

Epitope: first cysteine-rich ligand binding domain

a trypic digest of bovine low density lipoprotein receptor

Application

ELISA (0.5-1 g/ml)
Flow Cytometry (see application references)
Frozen Sections (1-5 g/ml)
Immunoblotting (1-5 g/ml, see application references)
Immunocytochemistry (1-5 g/ml, IF)
Immunoprecipitation (1-2 g/sample)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Positive Control
Liver sections, HT1080 cells, HepG2 cells

Other Notes

Will displace 50-70% of LDL binding to the receptor. For immunoblotting, include 2 mM Ca⁺² with the antibody and avoid EDTA. This antibody has been reported to bind best to the unreduced LDL-R (see specificity application reference). Antibody should be titrated for optimal results in individual systems.

van Driel, I.R., et al. 1987. J. Biol. Chem.262, 16127.
van Driel, I.R., et al. 1987. J. Biol. Chem.262, 17443.
Russell, D.W., et al. 1984. Cell37, 577.
Achneider, W.J., et al. 1983. J. Cell Biol.97, 1635.
Cummings, R.D., et al. 1983. J. Biol. Chem.258, 15261.
Schneider, W.J., et al. 1982 J. Biol. Chem.257, 2664.
Goldstein, J.L., et al. 1979. Nature279, 679.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg