MABE951

Anti-Histone H3.3 (H3-3A,H3-3B) Antibody

rat monoclonal, 6C4A3

• Synonym(s): Histone H3.3, H3.3A, H3F3
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: 6C4A3, monoclonal
• Species reactivity: human, monkey, mouse, canine
• Application: WB
• Citations: 1

Properties

• Product Name: Anti-Histone H3.3 Antibody, clone 6C4A3, clone 6C4A3, 1 mg/mL, from rat
• biological source: rat
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 6C4A3, monoclonal
• species reactivity: human, monkey, mouse, canine
• concentration: 1 mg/mL
• technique(s): western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_002098
• UniProt accession no.: P84243
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... H3F3B(3021)

Description

General description

Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the ′beads on a string′ structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

~17 kDa observed

Application

Anti-Histone H3.3 Antibody, clone 6C4A3 is an antibody against Histone H3.3 for use in western blotting.

Western Blotting Analysis: 0.1 µg/mL from a representative lot detected Histone H3.3 with recombinant H3.3 proteins. Additionally, 1 µg/mL from a representative lot detected Histone H3.3 in HeLa, COS1, NIH/3T3, NRK, and MDCK whole cell lysates (Prof. Taro Tachibana, Cell Engineering Corporation.).

Physical form

Format: Purified

Analysis Note

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1 µg/mL of this antibody detected Histone H3.3 in 10 µg of HeLa acid extract.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1