MAC131M

Mouse anti-Camelid IgG2/3 (heavy chain) Antibody, clone P17JE11

clone P17JE11, from mouse

• Synonym(s): HCAbs, Camelid antibody
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: P17JE11, monoclonal
• Species reactivity: Camelidae, camel, llama
• Application: ELISA
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: P17JE11, monoclonal
• species reactivity: Camelidae, camel, llama
• packaging: antibody small pack of 25 μg
• technique(s): ELISA: suitable
• isotype: IgG1κ
• target post-translational modification: unmodified

Description

General description

Camelidae family that includes camels and llamas are the only mammals reported to have heavy-chain (IgG-like) antibodies. Camelids produce conventional antibodies, but they also produce antibodies that do not associate with light chains. They produce functional antibodies devoid of light chains of which the single N-terminal domain is fully capable of antigen binding. Their VHH domain is located on a single stretch of amino acids and retains its antigen-recognition capability. Their heavy chains are devoid of the CH1 domain and have modified VH domain. Camelid heavy-chain antibodies (HCAbs) comprise about 50% of serum IgG in Llama and about 75% in Camelus species. HCAbs exhibit a molecular mass of about 100 kDa, which is smaller than conventional IgG1 and allows it to bind epitopes that may not be accessible to conventional antibodies. Also, the smaller size and single domain make it easier to transform them into bacterial cells for larger production needs. HCAbs are reported to display similar specificities, but are more robust in some cases. (Ref.: Daley LP et al. (2010). Clin. Vaccine Immunol. 17(2); 239-246; Harmsen, MM., and De Haard, HJ (2007) Appl Microbiol Biotechnol 77(1):13-22).

Immunogen

Camelid heavy-chain immunoglobulin G (clgG2/3).

Application

Mouse anti-Camelid IgG2/3 (heavy chain), clone P17JE11 detects Camelid IgG2/igG3 heavy chain. It has been tested for use in ELISA.

Research Sub Category
Secondary & Control Antibodies

Biochem/physiol Actions

Clone P17JE11 detects Camelid IgG2/igG3 heavy chain. Displays trivial to none reactivity with other species.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by ELISA in IgG as tested in various species.

ELISA Analysis: 2 µg/mL of this antibody detected Camelid IgG2/3 (heavy chain).

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1