SCC574

3dGRO^® Human Breast Organoids (DS117T)

Infiltrating Ductal Carcinoma, Breast, Age: 84, Female, Mutation: Notch

• Synonym(s): 3dGRO^® Human Breast Organoids (DS117T), Breast Cancer Organoid, Breast Cancer PDO, Breast Tumor Organoid, Human Breast Tumor Organoid, Mammary Organoids, TNBC Organoid, Triple-Negative Breast Cancer Organoid
• UNSPSC Code: 41106514
• NACRES: NA.61

Properties

• Quality Segment: 100
• biological source: human breast
• packaging: vial of ≥1500 vial (viable cells per vial)
• manufacturer/tradename: Millipore
• gender: female
• technique(s): cell culture: suitable
• application(s): 3D cell culture
• control: breast
• relevant disease(s): carcinoma (Infiltrating Ductal)
• shipped in: liquid nitrogen
• storage temp.: −196°C

Description

General description

Triple-negative breast cancer (TNBC) patient-derived organoids (PDOs) are advanced 3D in-vitro models that accurately recapitulate the genetic, phenotypic, and functional characteristics of TNBC tumors. TNBC is an aggressive and heterogeneous breast cancer subtype defined by the absence of estrogen receptor (ER), progesterone receptor (PR), and HER2 amplification, making it unresponsive to endocrine and HER2-targeted therapies. TNBC PDOs are established from patient tumor biopsies or resections and cultured in an extracellular matrix (ECM)-rich environment using optimized organoid media. TNBC PDOs provide a physiologically relevant alternative to 2D cultures and PDX models, retaining key tumor hallmarks such as high proliferation, Intratumoral Heterogeneity (ITH), invasiveness, genomic instability, and therapy resistance. Intratumoral heterogeneity in these organoid models enable accurate tumor modeling, drug response prediction, and biomarker discovery for advanced screening and personalized medicine.

Breast cancer (BC) is the most frequently diagnosed cancer in women worldwide, making up around 32% of all new cancer cases. BC includes over 21 distinct histological subtypes and four major molecular subtypes: Luminal A, Luminal B, HER2-positive, and the highly aggressive TNBC subtype. TNBC is a subtype of invasive ductal carcinoma (IDC), a prevalent type of BC, and represents approximately 10-20% of all BC cases. TNBC comprises several distinct molecular subtypes, each with unique biological characteristics and clinical implications

Basal-like (BL1 and BL2): Basal-like Immune-Activated (BLIA): In contrast to BLIS, BLIA shows better clinical outcomes, likely due to immune activation within the tumor microenvironment

Basal-like Immunosuppressed (BLIS): This subtype is marked by chromosomal instability and a homologous recombination deficiency (HRD) mutation signature, highlighting the potential for targeted therapies based on DNA repair mechanisms.

• These subtypes show elevated expression of genes involved in DNA damage repair and cell cycle regulation, often indicating an increased sensitivity to DNA-damaging therapies.
• Mesenchymal (M) and Mesenchymal Stem-like (MSL): Defined by high expression of genes associated with epithelial-mesenchymal transition (EMT) and growth factor signaling, these subtypes are often linked to increased metastasis and poor prognosis.
• Luminal Androgen Receptor (LAR): Characterized by prominent androgen receptor signaling, the LAR subtype may respond to androgen receptor-targeted therapies, offering potential therapeutic avenues.
• The 3dGRO^® Triple-Negative Breast Cancer (TNBC) Organoid biobank offers a selection of patient-derived TNBC organoids, for advanced disease modeling research. This biobank includes highly characterized organoid lines from different donors, representing basal-like metastatic TNBCs (BL-mTNBCs). Our organoid lines exhibit expression of both basal and luminal markers, highlighting their molecular heterogeneity and luminal progenitor lineage.

Cell Line Characteristic

• Sex: Female
• Age: 84 Years
• Race: African American
• Organ: Breast (Primary Tumor).
• Disease: Triple-Negative (ER-/ PR-/ HER2-) Invasive Ductal Carcinoma (Grade 3). Mutations: Notch

Application

• Viability: >1500 viable organoids/vial
• Organoid Growth: Pass
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Mycoplasma Contamination: Negative
• STR Profile: Pass

Features and Benefits

Cryopreserved patient derived triple-negative (ER-, PR-, HER2-) breast tumor organoids (PDOs).

Preparation Note

Store in liquid nitrogen. The organoids can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Legal Information

3dGRO^® is a trademark of Merck KGaA, Darmstadt, Germany.
3dGRO^® organoids were derived utilizing HUB Organoid Technology.

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/pdouse. This product may not be re-engineered or copied; used to make derivatives or modifications; used to develop, test, or manufacture a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact organoids@milliporesigma.com.

3dGRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product is regulated in France when intended for scientific purposes, including import, export, and conservation activities (Articles L.1211-1, paragraph 2, and L.1243-4 of the French Public Health Code). The purchaser (i.e. end user) is required to obtain an import authorization from the France Ministry responsible for Research (as referenced in Article L.1245-5-1 II of the French Public Health Code) or a conservation authorization from the same ministry (as referenced in Article L.1243-4 of the French Public Health Code). By placing an order for this product, you confirm that you have obtained the appropriate import or conservation authorization for the product. For more information regarding the use of human biological samples for research purposes in France, please contact the relevant French authorities.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): No data available
• Flash Point (°C): No data available