SCT024

BioTracker 543 Yellow β-Gal Dye

Live cell imaging dye for β-galactosidase (β-gal) used to detect LacZ reporter gene expression and cellular senescence (SA-β-gal).

• Synonym(s): Live cell imaging probe
• UNSPSC Code: 12352207
• NACRES: NA.47
• eCl@ss: 32161000

Properties

• technique(s): cell based assay: suitable, live cell imaging : suitable
• detection method: fluorometric
• shipped in: ambient
• storage temp.: -10 to -25°C

Description

General description

β-galactosidase, also called beta-gal or β-gal, is a glycoside hydrolase enzyme that catalyzes the hydrolysis of β-galactosides into monosaccharides through the breaking of a glycosidic bond. β-gal is commonly used in molecular biology as a reporter marker to monitor gene expression using the chromogenic X-Gal. β-gal has also been used to measure cellular senescence (SA-β-gal).

The BioTracker 543 Yellow β-Gal Dye is a fluorescent probe for the detection of β-galactosidase in living cells. It can be applied to fluorescent imaging and selection of cell and tissue transfected with lacZ along as a measurement of cellular senescence. Other applications include gene analysis by fluorescent imaging, monitoring transfection efficiency, and the study of gene promoter or enhancer elements. Because the dye is almost non-fluorescent in the absence of β-galactosidase, this probe exhibits good S/N ratio.

Spectral Properties
Absorbance: 525nm (488nm compatible)
Emission: 543nm

Preparation Note

Store BioTracker 543 Yellow β-Gal Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable