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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 4 x 25 μg | Please contact Customer Service for Availability | CZK 4,180.00 | |
| 4 x 100 μg | Please contact Customer Service for Availability | CZK 12,300.00 |
CZK 4,180.00
Please contact Customer Service for Availability
recombinant
expressed in Pichia pastoris
assay
95% (gel filtration)
form
lyophilized
specific activity
≥150 units/mg protein
packaging
pkg of 4 × 100 μg (03708969001), pkg of 4 × 25 μg (03708985001)
manufacturer/tradename
Roche
storage temp.
−20°C
General description
This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.
Application
Biochem/physiol Actions
Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.
Preparation Note
Stabilizers: Ca2+ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α2-macroglobulin, α1-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!
Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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This Item | |||
|---|---|---|---|
| specific activity ≥150 units/mg protein | specific activity ≥10,000 units/mg protein | specific activity ≥10,000 units/mg protein | specific activity - |
| assay 95% (gel filtration) | assay - | assay - | assay - |
| recombinant expressed in Pichia pastoris | recombinant expressed in Pichia pastoris | recombinant expressed in Pichia pastoris | recombinant - |
| form lyophilized | form lyophilized powder | form lyophilized powder | form lyophilized (salt-free) |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| manufacturer/tradename Roche | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename Roche |
signalword
Danger
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
hcodes
Hazard Classifications
Eye Irrit. 2 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 3708985001 | 04061831833720 |
| 3708969001 | 04061831833713 |