UPRT

Ultra pure High-Temp Reverse Transcriptase

DNA-free, Nuclease-free high-temp RT, designed for further manufacture

Properties

• Quality Segment: 400
• recombinant: expressed in E. coli Expressed in, expressed in E. coli expressed in
• form: liquid
• feature: DNA free, RNase and DNase free (also Nickase free)
• packaging: pkg of 40 kU
• concentration: 200 unit/μL, 200 units/μL
• technique(s): RT-PCR: suitable, cDNA synthesis: suitable
• shipped in: dry ice
• storage temp.: −20°C

Description

General description

M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase DNA polymerase uses DNA, single-stranded RNA, or an RNA:DNA hybrid (using a primer) to synthesize a complementary DNA strand. Wild-type and traditional Moloney Murine Leukemia Virus Reverse Transcriptases (MMLV-RT) are restricted to lower temperatures (up to 50℃) during cDNA synthesis. For many reverse transcription reactions, temperatures higher than 50℃ are beneficial. Higher temperatures reduce the chances of inhibition due to RNA secondary structures, while at the same time increasing primer binding specificity. These benefits lead to an increase in desired cDNA yield from the reverse transcription reaction. Ultra pure High-temp Reverse Transcriptase maintains its performance at high temperatures and outcompetes wild-type MMLV-RTs.Ultra pure High-temp Reverse Transcriptase is manufactured under stringent conditions to ensure that it is free of detectable levels of DNA, DNase, RNase, and Nickase to reduce the risk of false positive and false negative results in molecular diagnostic assays. Ultra pure High-temp Reverse Transcriptase should be used with other ultra pure DNA-free reagents, such as Ultra pure 10x Reverse Transcriptase Buffer and Ultra pure DNA-free Water, to minimize false positive and negative results and improved cDNA synthesis.Ultra pure High-temp Reverse Transcriptase was designed for use in highly-regulated industries in applications that require a high level of performance, lot-to-lot consistency, and high sensitivity without false results from reagent-borne contamination while meeting industry-driven critical regulations. To meet this requirements we have increased the quality level of our products. The M-Clarity Program defines product quality. It provides transparency so that you can choose, with confidence, suitable products that meet your needs with respect to quality and regulatory compliance, transparency, change notification, and documentation.

Unit Definition-One unit incorporates 0.1 nmol of TTP into double stranded product in 10 min at 37°C using poly(A):oligo dT as a template:primer evaluated with a fluorescent assay.

Application

ApplicationsUltra pure High-temp Reverse Transcriptase can be used for:

• Reverse transcriptionFirst strand cDNA synthesis (up to 7 kb)
• Preparation of cDNA libraries
• One- or two-step RT-PCR and RT-qPCR
• Rapid Amplification of cDNA Ends (RACE)
• RNA-Seq
• For further manufacture

Ultra pure High-temp Reverse Transcriptase can be used for:

• Reverse transcription
• First strand cDNA synthesis (up to 7 kb)
• Preparation of cDNA libraries
• One- or two-step RT-PCR and RT-qPCR
• Rapid Amplification of cDNA Ends (RACE)
• RNA-Seq
• For further manufacture

Features and Benefits

• DNA-free
• DNase-free, RNase-free, and nickase-free
• Ideal for high throughput RT-PCR applications
• Compatible with Ultra pure High-temp Reverse Transcriptase
• Enhanced change control and quality agreement

Packaging

Ultra pure 10x Reverse Transcriptase Buffer is provided in larger SKU sizes for ease of use in high throughput RT-PCR applications and for further manufacture. Ultra pure 10x Reverse Transcriptase Buffer should be used with other ultra pure DNA-free reagents, such as Ultra pure High-temp Reverse Transcriptase and Ultra pure DNA-free Water, to minimize false positive and negative results.

Other Notes

Quality testing ensures the absence of detectable levels of contaminating DNA (≤ 1 copy of prokaryotic gDNA, ≤ 0.1 copy of eukaryotic gDNA, and ≤ 10 copies of plasmid DNA per 100 units of enzyme) as detected by qPCR using universal primers against 16S capable of detecting > 500,000 prokayotes, 18S capable of detecting > 110,000 eukaryotes, and Ori capable of detecting > 25,000 plasmids, respectively.

Why Choose Ultra Pure & DNA-Free High Temp RT Enzyme?

• High-Temperature Performance That Outcompetes Wild-Type MMLV-RT-

• Traditional MMLV Reverse Transcriptases are limited to temperatures of up to 50°C, which can compromise cDNA synthesis when RNA secondary structures are present. Our Ultra pure High-Temp RT Enzyme operates effectively beyond this threshold, disrupting inhibitory RNA secondary structures and improving primer binding specificity — directly translating to higher cDNA yields and more reliable results.
• Ultra pure & DNA-free for Sensitive Workflows- Manufactured under stringent quality conditions, our High-Temp RT Enzyme is rigorously tested and certified free of detectable levels of DNA, DNase, RNase and Nickase. This level of purity significantly reduces the risk of false positive and false negative results in molecular diagnostic assays, making it a reliable choice for high-sensitivity applications.
• Designed for Highly Regulated Industries- Our High-Temp RT Enzyme is purpose-built for industries where precision, consistency, and compliance are non-negotiable. It delivers:
• Lot-to-lot consistency for reproducible results across experiments and production runs
• High sensitivity without interference from reagent-borne contamination
• Compliance-ready performance suitable for regulated molecular diagnostic and research environments
• Backed by the M-Clarity Program- Every batch of our High-Temp RT Enzyme is supported by the M-Clarity Program — a quality framework that provides full transparency across product quality, regulatory compliance, change notification, and documentation. This gives you the confidence to select and use our products knowing they meet your regulatory and quality requirements.
• Broad Application Versatility- Our High-Temp RT Enzyme is validated across a wide range of RNA-based workflows, including:
• First strand cDNA synthesis (up to 7 kb)
• One- and two-step RT-PCR and RT-qPCR
• Preparation of cDNA libraries
• Rapid Amplification of cDNA Ends (RACE)
• RNA-Seq
• High-throughput RT-PCR applications
• Further manufacture
• Part of a Complete Ultra pure Ecosystem- For optimal results, our High-Temp RT Enzyme is designed to work seamlessly with other Ultra pure DNA-free reagents, including Ultra pure 10x Reverse Transcriptase Buffer (UPRTBF)and Ultra pure DNA-free Water (UPH2O). Using matched ultra-pure reagents across your workflow minimizes contamination risk at every step, ensuring the integrity of your cDNA synthesis from start to finish.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable