11012002

KYAE-1 cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Morphology: Epithelial
• Receptors: Not specified
• Technique(s): cell culture | mammalian: suitable

Properties

• biological source: human esophagus
• Quality Segment: 100
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 11012002-DNA-5UG, pkg of vial of cells 11012002-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: P53 mutation c.455C>T
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer

Description

Biochem/physiol Actions

Distal oesophageal adenocarcinoma,

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 11
D5S818: 10,13
D7S820: 11,12
THO1: 6
TPOX: 8
vWA: 14

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Preparation Note

RPMI-1640:Hams F12 1:1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. There is a requirement to change the culture medium every 2-3 days.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable