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22178

Cellulase from Aspergillus niger

powder, off-white, ~0.8 U/mg

Synonym(s):

1,4-(1,3:1,4)-β-D-Glucan 4-glucanohydrolase

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Size/SKUAvailabilityPrice
25 g
Please contact Customer Service for Availability
3 990,00 CZK
100 g
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13 200,00 CZK

About This Item

CAS Number:
9012-54-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-734-4
MDL number:
MFCD00081510
EC Number:
3.2.1.4 (BRENDA, IUBMB)
eCl@ss:
32160410
Specific activity:
~0.8 U/mg
Biological source:
Aspergillus niger

3 990,00 CZK

Please contact Customer Service for Availability
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biological source

Aspergillus niger

Quality Segment

form

powder

specific activity

~0.8 U/mg

color

off-white

storage temp.

2-8°C

General description

Cellulase is a member of glycoside hydrolase family and is produced by a number of cellulolytic microbes.

Application

Cellulase from Aspergillus niger has been used:
  • in the preparation of the standardized solution to determine absolute cellulase levels
  • in phosphate-citric acid buffer for activity assay
  • in non-starch polysaccharide degrading enzyme cocktail for in vitro hydrolysis of defatted rice bran and in the approach to improve the nutritive value of poultry feed

Biochem/physiol Actions

Cellulase from Aspergillus niger catalyzes the hydrolysis of endo-1,4-β-D-glycosidic linkages in cellulose, lichenin, barley glucan, and the cellooligosaccharides cellotriose to cellohexaose. It does not cleave cellobiose or p-nitrophenyl-β-D-glucoside. This enzyme will also cleave intact glycosaminoglycan from a core peptide by hydrolyzing the xylosyl serine linkage.

Other Notes

1 U corresponds to the amount of enzymes which liberates 1 μmol glucose from carboxymethylcellulose per minute at pH 5.0 and 37°C
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

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This Item
C1184H2125A1602
Sigma-Aldrich

Sigma-Aldrich

22178

Cellulase from Aspergillus niger

Cellulase from Aspergillus niger powder, ≥0.3 units/mg solid

Sigma-Aldrich

C1184

Cellulase from Aspergillus niger

Hemicellulase from Aspergillus niger powder, 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate)

Sigma-Aldrich

H2125

Hemicellulase from Aspergillus niger

Amyloglucosidase from Aspergillus niger ammonium sulfate suspension, ≥40 units/mg protein

Sigma-Aldrich

A1602

Amyloglucosidase from Aspergillus niger

description

powder, off-white, ~0.8 U/mg

description

powder, ≥0.3 units/mg solid

description

powder, 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate)

description

ammonium sulfate suspension, ≥40 units/mg protein

biological source

Aspergillus niger

biological source

-

biological source

-

biological source

-

specific activity

~0.8 U/mg

specific activity

≥0.3 units/mg solid

specific activity

0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate)

specific activity

≥40 units/mg protein

form

powder

form

powder

form

powder

form

ammonium sulfate suspension

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

color

off-white

color

-

color

-

color

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

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    Certificates of Analysis (COA)

    Lot/Batch Number
    BCCN1094
    BCCP7731
    1291341
    1214145
    BCBR4363V

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