85639

SSC Buffer

for Northern and Southern blotting, powder blend

• Synonym(s): Saline sodium citrate buffer dry blend
• NACRES: NA.25
• UNSPSC Code: 12161700

Properties

• grade: Molecular Biology
• Quality Segment: 100
• product line: BioReagent
• form: powder blend
• technique(s): nucleic acid detection: suitable
• impurities: DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected
• solubility: H₂O: 0.263 g/mL, clear, colorless
• storage temp.: room temp

Description

General description

SSC Buffer powder is a standard reagent in Southern and Northern blotting procedures. This powder can be used to make SSC working solutions with concentrations ranging from 0.5x to 20x.

Application

Suitable for:

• Preparing nucleic acids for hybridization
• Hybridization buffer for Northern and Southern blots
• Washing buffer for Northern and Southern blots

Preparation Note

• Convenient pack sizes
• Multi-purpose use in hybridizations
• Easy-to-use powder makes fast, inexpensive solutions

Dissolve 263.5 g in a final volume of 1L molecular biology grade water. The resulting 20x concentrate is approximately pH 7, and can be further diluted with water, as needed.

Other Notes

SSC Buffer powder contains 0.3 M sodium citrate in 3M NaCl.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)