94403

Hoechst 33258 solution

1 mg/mL in H₂O, ≥98.0% (HPLC)

• Synonym(s): bisBenzimide H 33258 solution
• Empirical Formula (Hill Notation): C₂₅H₂₄N₆O · 3HCl
• CAS Number: 23491-45-4
• Molecular Weight: 533.88
• MDL number: MFCD29074011
• UNSPSC Code: 12171500
• PubChem Substance ID: 329770334
• NACRES: NA.32
• Assay: ≥98.0% (HPLC)
• Form: liquid

Properties

• Quality Segment: 100
• assay: ≥98.0% (HPLC)
• form: liquid
• mol wt: 533.88 g/mol
• concentration: 1 mg/mL in H₂O
• color: colorless, very light green-yellow to dark green-yellow, very light yellow to dark yellow
• pH: 6.0-8.0 (25 °C)
• solubility: DMF: soluble, H₂O: soluble
• density: 1.00 g/cm³
• fluorescence: λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• storage temp.: 2-8°C
• SMILES string: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
• InChI key: SMNPLAKEGAEPJD-UHFFFAOYSA-N

Description

General description

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

Application

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

Safety Information

• Storage Class: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable