A0855

Anti-Auxin antibody, Mouse monoclonal

clone 1E11-C11, purified from hybridoma cell culture

• Synonym(s): Anti-IAA, Anti-Indole-3-acetic acid
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD07785405
• Conjugate: unconjugated
• Clone: 1E11-C11, monoclonal
• Application: DB, ELISA (i), IF, IHC, WB
• Citations: 15

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in mouse cell line
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 1E11-C11, monoclonal
• form: PBS solution
• species reactivity: plant
• technique(s): dot blot: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, indirect ELISA: 1-2 μg/mL using using IAA conjugated to BSA, western blot: suitable
• isotype: IgG2b
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Auxin is a plant hormone that regulates physiological growth and development. This hormone regulates cellular functions such as elongation, turgor, division, and differentiation. Indoleacetic acid (IAA) is recognized as the main auxin in several plants. IAA is synthesized from tryptophan (Trp) using Trp dependent pathways,. Mouse monoclonal anti-auxin antibody recognizes indoleacetic acid but not free unmethylated IAA.

Immunogen

indoleacetic acid (IAA) carboxyl linked to ovalbumin.

Application

Indole-3-acetic acid was identified in developing P.abies spruce plant parrafin embedded formalin-fixed embryos by immunohistochemistry with the monoclonal anti-indole-3-acetic acid antibody.

Mouse monoclonal anti-auxin antibody has been used for immunofluorescence studies. The antibody can also be used for ELISA, dot blot and western blot analyses.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)