A1233

AMPK (α1/β1/γ1), active, His tagged human

PRECISIO^® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): AMPKG, HAMPKb, MGC17785, MGC33776, MGC57364, MGC8666, PRKAA1, PRKAB1, PRKAG1
• NACRES: NA.32
• UNSPSC Code: 12352200
• Specific activity: 626-848 nmol/min·mg
• Assay: ≥70% (SDS-PAGE)
• Biological source: human
• Recombinant: expressed in baculovirus infected Sf9 cells

Properties

• biological source: human
• Quality Segment: 200
• recombinant: expressed in baculovirus infected Sf9 cells
• product line: PRECISIO^® Kinase
• assay: ≥70% (SDS-PAGE)
• form: buffered aqueous glycerol solution
• specific activity: 626-848 nmol/min·mg
• mol wt: A1 subunit ~68 kDa, B1 subunit ~38 kDa, G1 subunit ~40 kDa
• solubility: water: soluble
• UniProt accession no.: P54619, Q13131, Q9Y478
• application(s): life science and biopharma
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... PRKAA1(5562), PRKAB1(5564), PRKAG1(5571)

Description

General description

Research area: CELL SIGNALING

AMP-activated protein kinase (AMPK) is a heterotrimer protein kinase consisting of a α catalytic subunit, and non-catalytic β and γ subunits.

Application

AMP-activated protein kinase (AMPK) has been used to access the binding kinetics of thalidezine to recombinant active human AMPK using Bio-Layer Interferometry (BLI) binding assay.

Biochem/physiol Actions

AMP-activated protein kinase (AMPK) is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated and phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and 3-hydroxy-3-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating biosynthesis of fatty acid and cholesterol.

Physical form

Supplied in 50mM NaPhosphate pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1mM PMSF, 0.2 mM DTT, 25% glycerol.

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Codes: H315,H319,H360D
• Precautionary Statements: P202 - P264 - P280 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter