An essential product for immunoprecipitations of VSV-tagged recombinant proteins. Given the packaging of the product, we aliquoted it into microfuge tubes on arrival and stored it at 4 C.
A1970
Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal
clone P5D4, purified from hybridoma cell culture, PBS suspension
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
Monoclonal Anti-VSV Glycoprotein
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Please contact Customer Service for Availability | 17 200,00 CZK |
About This Item
NACRES:
NA.56
UNSPSC Code:
12352203
Conjugate:
agarose conjugate
Clone:
P5D4, monoclonal
Application:
IP, protein purification
Citations:
7
17 200,00 CZK
Please contact Customer Service for Availability
biological source
mouse
Quality Segment
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P5D4, monoclonal
form
PBS suspension
analyte chemical class(es)
proteins (VSV-G)
technique(s)
immunoprecipitation (IP): suitable, protein purification: suitable
isotype
IgG1
capacity
≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
shipped in
wet ice
storage temp.
2-8°C
General description
Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.
Immunogen
synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.
Application
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.
Biochem/physiol Actions
The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.
Physical form
Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Preparation Note
Prepared using cyanogen bromide-activated agarose.
1 of 1
This Item | |||
|---|---|---|---|
| description clone P5D4, purified from hybridoma cell culture, PBS suspension | description clone P5D4, purified from hybridoma cell culture | description affinity isolated antibody, buffered aqueous solution | description affinity isolated antibody, buffered aqueous solution |
| antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| conjugate agarose conjugate | conjugate - | conjugate FITC conjugate | conjugate unconjugated |
| biological source mouse | biological source mouse | biological source goat | biological source goat |
| clone P5D4, monoclonal | clone P5D4, monoclonal | clone polyclonal | clone polyclonal |
| technique(s) immunoprecipitation (IP): suitable, protein purification: suitable | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, immunoblotting: 0.5-1 μg/mL using whole extract of human HEK-293T cells over-expressing Vinculin with VSV-G tagged fusion protein., immunofluorescence: 5-10 μg/mL using COS7 cells over-expressing Vinculin with VSV-G tagged fusion protein. | technique(s) direct immunofluorescence: 1:64 | technique(s) indirect ELISA: 1:10,000 |
| shipped in wet ice | shipped in dry ice | shipped in - | shipped in dry ice |
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Storage Class
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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