A3449

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 19:1

• Synonym(s): 30% Acrylamide/Bisacrylamide 19:1, Polyacrylamide solution
• UNSPSC Code: 41105319
• PubChem Substance ID: 329770824
• NACRES: NA.25
• MDL number: MFCD00080848

Properties

• Product Name: Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 19:1
• sterility: 0.2 μm filtered
• Quality Segment: 200
• product line: BioReagent
• form: liquid
• feed ratio: 19:1
• technique(s): electrophoresis: suitable
• suitability: suitable for electrophoresis
• foreign activity: Protease, none detected
• storage temp.: 2-8°C
• SMILES string: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• InChI key: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

Safety Information

• Pictograms: GHS08,GHS07
• Signal Word: Danger
• Hazard Codes: H302 + H332,H315,H317,H319,H340,H350,H361f,H372
• Precautionary Statements: P280 - P301 + P312 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Target Organs: Peripheral nervous system
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter