A3547

Achromopeptidase from bacteria

lyophilized powder, ≥1,000 units/mg solid

• Synonym(s): ACP, Lysyl endopeptidase
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥1,000 units/mg solid
• Biological source: bacterial (LYSOBACTER SP. BACTERIA M497-1)

Properties

• biological source: bacterial (LYSOBACTER SP. BACTERIA M497-1)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥1,000 units/mg solid
• mol wt: 27 kDa
• technique(s): cell based assay: suitable
• input: bacteria and yeast sample(s)
• suitability: suitable for cell lysis
• application(s): diagnostic assay manufacturing
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections.

Biochem/physiol Actions

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Achromopeptidase is a lysyl endopeptidase with a MW of ~27 kDa. It is useful for lysis of Gram-positive bacteria that are resistant to lysozyme.
pH Optimum for activity: pH 8.5 - 9
Approximately 500-1,500 un/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀=0.6 over 2 hours at 37 °C.

Physical form

crude powder

Other Notes

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H334
• Precautionary Statements: P261 - P342 + P311
• Hazard Classifications: Resp. Sens. 1
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• PPE (personal protective equipment): dust mask type N95 (US), Eyeshields, Faceshields, Gloves