A5403

Anti-Human IgG (γ-chain specific), F(ab′)₂ fragment−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.77
• MDL number: MFCD00162443
• Conjugate: alkaline phosphatase conjugate
• Clone: polyclonal
• Application: ELISA (d)
• Citations: 8

Properties

• biological source: goat
• Quality Segment: 200
• conjugate: alkaline phosphatase conjugate
• antibody form: affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• technique(s): direct ELISA: 1:1,000
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Human IgG antibodies are primarily responsible for the facilitation of humoral immune responses such as placental transport, complement fixation and phagocytosis among many others. The antigen binding is mediated by the variable Fab region of IgG antibodies, whereas the effector activities are stimulated by the Fc domain , . Anti-human IgG (γ-chain specific), F(ab′)₂ fragment-alkaline phosphatase antibody can be used in ELISA for measuring anticardiolipin antibodies. Goat anti-human IgG (γ-chain specific), F(ab′)₂ fragment-alkaline phosphatase antibody reacts specifically with human IgG.

Immunogen

Purified human IgG.

Application

Serum IgGs against HHV-6 or HHV-7 were detected by Elisa using alkaline phosphatase conjugated goat anti-human IgG F′ab specific at a concentration of 1:1000 diluted in PBS/0.05% skim milk. The antibody was incubated on plates for 2 hours at 37 degrees.

Transfected SN56 neuronal cell lysates were ran on a SDS page gel for western blot analysis using alkaline phosphatase conjugated goat anti-human IgG Fab specific as the secondary antibody.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable