The expiration date of Accuspin tubes is stated on the box, andits not possible to specify a shorter shelf life after opening. Individual Accuspin tube is sterile until the cap is removed, and the opened box or tube stability can only be determined by the user.
A6929
ACCUSPIN™ System-Histopaque®-1077
sterile-filtered, density: 1.077 g/mL
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Quality Level
sterility
aseptically filled
form
solution
shelf life
Expiry date on the label.
packaging
pkg of packaging 40 tubes
technique(s)
cell culture | mammalian: suitable
density
1.077 g/mL
application(s)
hematology
histology
storage temp.
2-8°C
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This Item | A7054 | 10771 | A0561 |
|---|---|---|---|
| form solution | form solution | form liquid | form solution |
| sterility aseptically filled | sterility aseptically filled | sterility sterile-filtered | sterility aseptically filled |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| density 1.077 g/mL | density 1.077 g/mL | density 1.077 | density 1.077 g/mL |
| Quality Level 400 | Quality Level 400 | Quality Level 400 | Quality Level 400 |
| packaging pkg of packaging 40 tubes | packaging pkg of package 12 tubes | packaging - | packaging package of 100 tubes |
General description
ACCUSPIN System-Histopaque-1077 products are intended for use in the isolation of lymphocytes and other mononuclear cells. The separation medium, Histopaque-1077, is a sterile-filtered, endotoxin-tested solution of polysucrose and sodium diatrizoate, adjusted to a density of 1.077 g/mL. ACCUSPIN System-HISTOPAQUE-1077 employs γ-irradiated 12mL polypropylene centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). Each tube is aseptically filled with 3 mL of Histopaque 1077. Each tube will separate 3-6 mL of anticoagulated blood.
Application
ACCUSPIN System-HISTOPAQUE-1077 employs centrifuge tubes specially designed with two chambers separated by a porous high-density polyethylene barrier ("frit"). The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
ACCUSPIN System-Histopaque-1077 is used for the separation of lymphocytes and other mononuclear cells from whole blood and bone marrow using density gradient separation media. Histopaque-1077 has been used for:
- human lymphocyte antigen (HLA) typing
- initial isolation step prior to the enumeration of T, B, and ‘null’ lymphocytes.
- preparation of pure lymphocyte suspensions for cell culture and cytotoxicity assays.
- isolation and purification of peripheral blood mononuclear cells (PBMCs) and bone marrow mononuclear cells (BMMCs)citation
- isolation of human lymphocytescitation
- isolation of breast cancer circulating tumor cells (CTCs) from the whole bloodcitation
Biochem/physiol Actions
The lower chamber contains HISTOPAQUE-1077 which allows the addition of anticoagulated whole blood without risk of mixing with the separation medium. On centrifugation, the whole blood descends through the frit to contact with the HISTOPAQUE-1077 below the frit, giving a clear separation of the blood components. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, and resulting in pelleting at the bottom of the ACCUSPIN tube. Lymphocytes and other mononuclear cells, i.e., monocytes, remain at the plasma-HISTOPAQUE-1077 interface.
Features and Benefits
ACCUSPIN™ System-Histopaque®-1077 offers numerous advantages, such as:
- Optimal recovery of viable cells
- Permits selective separation of blood cell types
- Prevents cell distortion
- Preserves cell viability
- Minimal extraneous cell interference
- Reproducible performance from batch to batch
- Histopaque is sterile-filtered and endotoxin-tested
- Stable at 2-8°C for at least 2 years when protected from light
Preparation Note
Package contains 40 prefilled tubes
Legal Information
Accuspin is a trademark of Sigma-Aldrich Co. LLC
Histopaque is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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