Skip to Content
Merck

Key Documents

View All Documentation

Skip To

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Synonym(s):

L-Alanine: NAD+ oxidoreductase (deaminating)

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View
Size/SKUAvailabilityPrice
100 units
Please contact Customer Service for Availability
8 570,00 CZK

About This Item

CAS Number:
9029-06-5
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-847-9
MDL number:
MFCD00131441
EC Number:
1.4.1.1 (BRENDA, IUBMB)
Specific activity:
~30 units/mg protein (Lowry)
Biological source:
Bacillus subtilis

8 570,00 CZK

Please contact Customer Service for Availability
​
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

biological source

Bacillus subtilis

Quality Segment

form

buffered aqueous glycerol solution

specific activity

~30 units/mg protein (Lowry)

foreign activity

LDH ~1% (using pyruvate as substrate)

storage temp.

−20°C

Application

L-Alanine dehydrogenase converts L-alanine to pyruvate and ammonium. L-Alanine dehydrogenase from Bacillus subtilis may be used to study enzyme inactivation and protection .

Biochem/physiol Actions

L-Alanine dehydrogenase is an A-stereospecific dehydrogenase that catalyzes the reversible deamination of L-alanine to pyruvate and ammonium. It is important for the generation of pyruvate during sporulation. L-Alanine dehydrogenase from Bacillus subtilis has a predominately ordered kinetic mechanism in which NAD binds before L-alanine. Subsequently, ammonia, pyruvate, and NADH are released in that specific order. Optimal pH for the amination reaction is 8.8-9.0, whereas it is 10-10.5 for the deamination reaction. The enzyme is inactivated by divalent metal ions and p-chloromercuribenzoate, mercuric ion being most effective. The inactivation may be reversed by L- or D-cysteine.

Physical form

Solution in 50% glycerol containing 10 mM potassium phosphate buffer, pH 7.7

Other Notes

One unit will convert 1.0 μmole of L-alanine to pyruvate and NH3 per min at pH 10.0 at 25 °C.

Compare Similar Items

View Full Comparison

Show Differences

1 of 1

This Item
A7189L5135431525
L-Alanine Dehydrogenase from Bacillus subtilis buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

Sigma-Aldrich

A7653

L-Alanine Dehydrogenase from Bacillus subtilis

L-Alanine Dehydrogenase from Bacillus subtilis ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

Sigma-Aldrich

A7189

L-Alanine Dehydrogenase from Bacillus subtilis

L-Leucine Dehydrogenase from Bacillus cereus lyophilized powder, ≥60 units/mg protein

Sigma-Aldrich

L5135

L-Leucine Dehydrogenase from Bacillus cereus

L-Leucine Dehydrogenase, Bacillus cereus

Sigma-Aldrich

431525

L-Leucine Dehydrogenase, Bacillus cereus

description

buffered aqueous glycerol solution, ~30 units/mg protein (Lowry)

description

ammonium sulfate suspension, ≥20 units/mg protein (Lowry)

description

lyophilized powder, ≥60 units/mg protein

description

-

biological source

Bacillus subtilis

biological source

Bacillus subtilis

biological source

-

biological source

Bacillus sp. (Bacillus cereus)

specific activity

~30 units/mg protein (Lowry)

specific activity

≥20 units/mg protein (Lowry)

specific activity

≥60 units/mg protein

specific activity

≥50 units/mL, ≥10 units/mg protein

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

liquid

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

foreign activity

LDH ~1% (using pyruvate as substrate)

foreign activity

-

foreign activity

-

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

Still not finding the right product?

Explore all of our products under L-Alanine Dehydrogenase from Bacillus subtilis

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

PPE (personal protective equipment)

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

Reviews

No rating value

Active Filters