A9207

Avidin−Agarose from egg white

Name: Avidin−Agarose from egg white
Brand: SIGMA
Price: 4240 CZK

aqueous glycerol suspension

Synonym(s):

Avidin agarose

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1 ML

CZK 4,240.00

5 ML

CZK 14,500.00

CZK 4,240.00

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About This Item

MDL number:

MFCD00130572

UNSPSC Code:

41106514

NACRES:

NA.56

Key Documents

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biological source

egg white

conjugate

agarose conjugate

form

aqueous glycerol suspension

matrix

cross-linked 6% beaded agarose

matrix activation

epichlorohydrin

matrix attachment

amino

matrix spacer

15 atoms

storage temp.

−20°C

Related Categories

Biotinylated Protein Purification

Protein Purification Chromatography

Protein Sample Prep

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Show Differences

1 of 4

This Item	A9275	189725	A2901
Sigma-Aldrich A9207 Avidin−Agarose from egg white	Sigma-Aldrich A9275 Avidin from egg white	Sigma-Aldrich 189725 Avidin, Egg White	Sigma-Aldrich A2901 Avidin–FITC from egg white
biological source egg white	biological source egg white	biological source -	biological source -
form aqueous glycerol suspension	form lyophilized powder	form lyophilized solid	form lyophilized powder
storage temp. −20°C	storage temp. 2-8°C	storage temp. −20°C	storage temp. −20°C
matrix cross-linked 6% beaded agarose	matrix -	matrix -	matrix -
conjugate agarose conjugate	conjugate -	conjugate -	conjugate FITC conjugate
Quality Level 200	Quality Level 200	Quality Level 100	Quality Level 200

General description

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.[1]

Application

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Avidin-Agarose from egg white has been used:

in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )[2]
in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni[3]
in avidin bead assay for purification of labelled biotin[4]
for capture of glycoproteins from murine cornea samples[5]

Biochem/physiol Actions

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications.[1] In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.[6]

Physical form

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

Other Notes

One unit will bind 1.0 μg of d-biotin.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Dielectric spectroscopy of molecular interactions based on the avidin-biotin complex

Mellor, B.L., et al.

Conference proceedings : Annual International Conference of the IEEE Engineering in Medicine and Biology Society, 3660-3663 (2011)

Corneal surface glycosylation is modulated by IL-1R and

Amber L Jolly et al.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(6), 2393-2404 (2017-02-23)

Cell surface glycosylation is thought to be involved in barrier function against microbes at mucosal surfaces. Previously we showed that the epithelium of healthy mouse corneas becomes vulnerable to

Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione

Kato Y, et al.

Redox Biology, 2, 983-990 (2014)

Characterization of poultry egg-white avidins and their potential as a tool in pretargeting cancer treatment

Hytonen VP, et al.

The Biochemical Journal, 372(1), 219-225 (2003)

Monolithic columns with immobilized monomeric avidin: preparation and application for affinity chromatography.

Jens Spross et al.

Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)

A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric

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Avidin−Agarose from egg white