A9792

Anti-Chicken IgY (IgG) (whole molecule)−Peroxidase antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Synonym(s): Rabbit Anti-Chicken IgY (IgG) (whole molecule)−HRP
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162225
• Conjugate: peroxidase conjugate
• Clone: polyclonal
• Application: DB, ELISA (d), IHC (p)
• Citations: 8

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: IgG fraction of antiserum
• antibody product type: secondary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: chicken
• technique(s): direct ELISA: 1:30,000, dot blot: 1:160,000 using indirect chemiluminescence system using 5 ng human IgG/dot and chicken anti-human IgG as the primary antibody and Luminol plus enhancer as the substrate., immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using using human pancreas sections and chicken anti-human insulin as the primary antibody.
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Chicken IgY is made of two heavy (H) and two light (L) chains. Its molecular weight is about ~180 kDa.

Immunogen

purified chicken IgY (IgG)

Application

Anti-Chicken IgY (IgG) (whole molecule) FITC antibody has been used in immunohistology.

Avian proteins over expressed and purified from E. coli were analyzed by western blot using HRP-conjugated rabbit anti-chicken IgY as the secondary at 1:2000 dilution.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody has been used for ELISA. The antibody can also be used for dot blot (1:160,000) and IHC (1:500) assays.

Biochem/physiol Actions

Chicken IgY is the major antibody found in chicken serum. Chickens generate a comparatively enhanced antibody response to mammalian antigenic proteins. These immunoglobulins do not bind to Fc receptors present in mammals and bacteria, and also do not interact with rheumatoid factors. Thus anti-chicken antibodies are useful analytical tools for various research applications.

IgY plays a major role in defense mechanism against systemic infections. It also possesses the ability to initiate anaphylactic reactions.

Rabbit Anti-Chicken IgY (IgG) (whole molecule)-Peroxidase antibody binds to chicken IgY.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Codes: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable