This product has not been tested on microalgae.
APOAC
Annexin V-Cy3™ Apoptosis Detection Kit
Synonym(s):
Red fluorescent Annexin V apoptosis assay
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Please contact Customer Service for Availability | 25 700,00 CZK |
About This Item
NACRES:
NA.32
UNSPSC Code:
12352207
eCl@ss:
32040201
25 700,00 CZK
Please contact Customer Service for Availability
usage
sufficient for 200 tests
Quality Segment
packaging
pkg of 1 kit
technique(s)
flow cytometry: suitable
application(s)
cell analysis
detection
detection method
fluorometric
shipped in
wet ice
storage temp.
2-8°C
Biochem/physiol Actions
Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).
Features and Benefits
Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
- Rapid labeling of cells. Cell staining takes only 10 minutes.
- No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
- 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.
Legal Information
Cy3 is a trademark of Cytiva
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This Item | |||
|---|---|---|---|
| description - | description - | description clone AN5, purified from hybridoma cell culture | description affinity isolated antibody, buffered aqueous solution |
| technique(s) flow cytometry: suitable | technique(s) flow cytometry: suitable | technique(s) western blot: 0.5-1 μg/mL using HeLa total cell extract, indirect ELISA: suitable, immunocytochemistry: suitable, microarray: suitable | technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50 |
| shipped in wet ice | shipped in wet ice | shipped in dry ice | shipped in wet ice |
| application(s) cell analysis | application(s) cell analysis | application(s) - | application(s) - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C |
| detection method fluorometric | detection method fluorometric | detection method - | detection method - |
| packaging pkg of 1 kit | packaging pkg of 1 kit | packaging - | packaging - |
Storage Class
10 - Combustible liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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can we use this kit used for microalgae cells
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