B6901

Monoclonal Anti-Bovine IgG antibody produced in mouse

clone BG-18, ascites fluid

• Synonym(s): Monoclonal Anti-Bovine IgG
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: unconjugated
• Clone: BG-18, monoclonal
• Application: DB, ELISA (i)
• Citations: 12

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: BG-18, monoclonal
• contains: 15 mM sodium azide
• species reactivity: bovine (non-reactive to IgM)
• should not react with: pig, human, rabbit, sheep, cat, guinea pig, canine, horse, goat
• technique(s): dot blot: suitable, indirect ELISA: 1:1000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

The antibody reacts with an epitope on both bovine IgG1 and IgG2 in their native form. The antibody shows no reaction with the epitope after denaturation and reduction. IgG (immunoglobulin G) contributes to 10–20% of plasma protein and is regarded as one of the most predominant serum protein. It consists of four subclasses: IgG1, IgG2, IgG3 and IgG4. The IgG structure possesses four polypeptide chains containing two identical γ heavy (H) chains and two identical γ or γ light (L) chains of 50 kDa and 25 kDa, respectively.

Immunogen

Purified bovine IgG

Application

Monoclonal Anti-Bovine IgG antibody has been used in indirect immunoperoxidase staining.

Monoclonal Anti-Bovine IgG antibody produced in mouse is suitable for ELISA at a working dilution of 1:20,000. It may be used for immunohistochemistry of formalin-fixed, paraffin-embedded sections at a dilution of 1:1000. Monoclonal Anti-Bovine IgG antibody produced in mouse was used as an isotype control in ChIP assay.

Biochem/physiol Actions

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.

bovine IgG1 and IgG2

Physical form

Ascites fluid containing 0.1% sodium azide

Preparation Note

For continuous use, store at 2-8°C. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable