C0887

Chloroperoxidase from Caldariomyces fumago

buffered aqueous suspension, 1,000-2,000 units/mg protein (E1%/280)

• Synonym(s): Chloride Peroxidase, Chloride:hydrogen-peroxide oxidoreductase
• CAS Number: 9055-20-3
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 1.11.1.10 (BRENDA, IUBMB)
• MDL number: MFCD00130774
• Specific activity: 1,000-2,000 units/mg protein (E1%/280)
• Biological source: fungus (Caldariomyces fumago)

Properties

• biological source: fungus (Caldariomyces fumago)
• Quality Segment: 200
• form: buffered aqueous suspension
• specific activity: 1,000-2,000 units/mg protein (E1%/280)
• mol wt: 42 kDa
• absorbance ratio: RZ ~1.0
• storage temp.: 2-8°C

Description

Application

A useful alternative to lactoperoxidase for ¹³¹I ion labeling studies, for bromination of proteins, and for ³⁶Cl labeling of macromolecules in long-term isolation procedures.

Biochem/physiol Actions

Chloroperoxidase (CPO) is a 42,000 Da extracellular heme glycoenzyme containing ferriprotoporphyrin IX as the prosthetic group. CPO is secreted from fungus and exhibits a broad spectrum of chemical reactivities. It is a peroxide-dependent chlorinating enzyme. It also catalyzes peroxidase-, catalase- and cytochrome P450-type reactions of dehydrogenation, H₂O₂ decomposition and oxygen insertion, respectively. The enzyme has magnetic and spectroscopic properties similar to that of cyctochrome P-450. CPO from the fungus Caldariomyces fumago has the capacity to chlorinate aromatic hydrocarbons, including polycyclic aromatic hydrocarbons (PAHs).

Physical form

Purified suspension in 0.1 M sodium phosphate solution, pH approx. 4.5

Other Notes

One unit will catalyze the conversion of 1.0 μmole of monochlorodimedon to dichlorodimedon per min at pH 2.75 at 25 °C in the presence of potassium chloride and H₂O₂.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)