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C2273

Carbonic Anhydrase from bovine erythrocytes

For use as a marker in SDS-PAGE

Synonym(s):

Carbonate Dehydratase, Carbonate Hydrolyase

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Size/SKUAvailabilityPrice
1 vial
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3 410,00 CZK

About This Item

CAS Number:
9001-03-0
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-576-6
MDL number:
MFCD00081467
EC Number:
4.2.1.1 (BRENDA, IUBMB)
eCl@ss:
32035490
Biological source:
bovine erythrocytes

3 410,00 CZK

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biological source

bovine erythrocytes

Quality Segment

form

powder

mol wt

~29 kDa

packaging

vial of 5 mg

storage temp.

2-8°C

General description

Carbonic Anhydrase is a metalloenzyme with zinc. The active site of carbonic anhydrase contains three conserved histidine residues (His94, His96 and His119).

Application

Carbonic Anhydrase has been used:
  • as a component of nine-protein mixture for the comparative proteomic mass spectrometry analysis of bovine brain samples
  • as a molecular marker in gel filtration chromatography
  • as a standard protein to test the recovery of CMA/20 microdialysis probe

Biochem/physiol Actions

Carbonic anhydrase is essential for the reversible hydration of carbon dioxide. It is also crucial for the physiological processes including respiration, ion transport, acid–base balance, lipid and carbohydrate metabolic pathways. Carbonic anhydrase participates in electrolyte secretion and in the carbon dioxide fixation.

Other Notes

One collagen digestion unit (CDU) liberates peptides from collagen from bovine achilles tendon equivalent in ninhydrin color to 1.0 μmole of leucine in 5 hours at pH 7.4 at 37 °C.

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Codes

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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Questions

1–2 of 2 Questions  

  1. Which isozyme of the C2273 Carbonic Anhydrase from bovine erythrocytes (CA I or CA II)?

    1 answer

    1. This product is intended for use as a molecular weight marker. The isoforms present have not been specifically identified. However, bovine erythrocytes typically show a higher concentration of CA II. The raw material used to produce this product has been shown to be predominantly CA II with lower concentrations of CA I and CA IV.

      Helpful?

  2. Does the bovine carbonic anhydrase product contain zinc ?

    1 answer

    1. The active site of most carbonic anhydrases contains a zinc ion. This enzyme is considered a zinc metalloenzyme, however testing is not performed to confirm the presence of zinc.

      Helpful?

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