C3260

Citrate Synthase from porcine heart

liquid, ≥100 units/mg protein

• Synonym(s): Citrate condensing enzyme, Citrate oxaloacetate lyase (CoA-acetylating)
• CAS Number: 9027-96-7
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 4.1.3.7 (BRENDA, IUBMB)
• MDL number: MFCD00130808
• Specific activity: ≥100 units/mg protein

Properties

• Quality Segment: 200
• form: liquid
• specific activity: ≥100 units/mg protein
• foreign activity: isocitrate dehydrogenase and aconitase ≤0.01%, malic dehydrogenase ≤0.1%
• storage temp.: −20°C

Description

Application

Citrate Synthase from porcine heart has been used:

• to inject newt egg for determining its importance in egg activation at fertilization
• in the preparation of reaction mix to determine pyruvate carboxylase enzyme activity
• to examine whether it can induce a [Ca²⁺] increase and egg activation in unfertilized eggs

Biochem/physiol Actions

Citrate synthase catalyses the conversion of citrate to acetyl-CoA in the presence of coenzyme-A with the release of H₂O and oxaloacetate. The enzyme has a molecular weight of 85 kDa and a pI of 6.1-6.6. It is inhibited by fluoroacetyl-CoA, palmitoyl-CoA, and citroyl-CoA. It is also inhibited when it is acetylated by acetic anhydride or iodinated by iodine.

Physical form

Suspension in 3.2 M (NH₄)₂SO₄ solution, pH 7.0.

Other Notes

One unit will form 1.0 μmole of citrate from oxalacetate and acetyl CoA per min at pH 8.0 at 37 °C.

Safety Information

• Storage Class: 12 - Non Combustible Liquids