C3535

Complement C8 from human serum

suitable for radioiodination, RIA, ≥85% (SDS-PAGE), ≥125,000 C8H50 units/mg protein (using C8 deficient serum)

• CAS Number: 80295-58-5
• UNSPSC Code: 12352202
• NACRES: NA.61
• MDL number: MFCD00130847
• Form: solution
• Assay: ≥85% (SDS-PAGE)
• Biological source: human

Properties

• biological source: human
• Quality Segment: 200
• assay: ≥85% (SDS-PAGE)
• form: solution
• specific activity: ≥125,000 C8H50 units/mg protein (using C8 deficient serum)
• concentration: 1 mg/mL in PBS, pH 7.2
• technique(s): immunoelectrophoresis: suitable
• suitability: suitable for radioiodination, RIA
• UniProt accession no.: P07357
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... C8A(731)

Description

Application

Complement C8 is one of the end terminals of the complement system contained in the membrane attack complex (MAC). In the (MAC), C8 initiates and coordinates MAC pore formation. It has been used in research to investigate the mechanisms of how C8-C9 and C9-C9 interactions facilitate folding of the membrane attack complex perforin (MACPF) domain to form the circular pore.

Biochem/physiol Actions

C8 is one of the components of the terminal complement complex and is required for a functional complement system. It is composed of disulfide-linked C8α and C8γ and a noncovalently associated C8β chain. During formation of the membrane-attack complex, a domain on C8α induces C9 to polymerize and form a pore-like structure.

Analysis Note

Functionally pure by a sensitive hemolytic assay using depleted sera.

Other Notes

View more information on the complement pathway at www.sigma-aldrich.com/enzymeexplorer

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable