C4483

CD152/Fc Chimera, Cytolytic from mouse

recombinant, expressed in NS.1 cells, buffered aqueous solution

• Synonym(s): CTLA4/Fc Chimera, cytolytic
• UNSPSC Code: 51111800
• NACRES: NA.77
• MDL number: MFCD01092888
• Form: buffered aqueous solution
• Assay: ≥99% (SDS-PAGE)
• Biological source: mouse
• Recombinant: expressed in NS.1 cells
• Mol wt: 97 kDa

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in NS.1 cells
• assay: ≥99% (SDS-PAGE)
• form: buffered aqueous solution
• mol wt: 97 kDa
• packaging: pkg of 1 mg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: ≤1 EU/mg protein Endotoxin level (LAL test)
• UniProt accession no.: P09793
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... Cd152(12477), Ctla4(12477)

Description

General description

The complement (C1q) and FcγR I binding sites of the Fcγ2a fragment allow the Fc portion of the fusion protein to effectively facilitate direct antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

Biochem/physiol Actions

CD152 (CTLA4), a cell surface glycoprotein expressed at low levels on activated T cells, is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2). A related cell surface glycoprotein, CD28, binds to CD80 and CD86 with lower affinity. The soluble CD152/Fc chimeric fusion protein blocks the B7/CD28 signaling pathway by binding to CD80 and CD86.

Physical form

Solution, 0.2 μm filtered, in phosphate buffered saline, pH 7.4, with no preservative added.

Preparation Note

Purified from serum-free tissue culture supernatant

Other Notes

The extracellular domain (160 amino acids) of mouse CD152 is fused to mouse IgG2a Fc domain.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)