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C5491

Cellytic™ Express, 1 mL Tablets

1 tablet sufficient for 1 mL lysis (cell suspension)

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Size/SKUAvailabilityPrice
25 ea
Please contact Customer Service for Availability
2 540,00 CZK
100 ea
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7 580,00 CZK

About This Item

NACRES:
NA.56
UNSPSC Code:
41105500

2 540,00 CZK


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form

tablet

Quality Segment

usage

 tablet sufficient for 1 mL lysis (cell suspension)

input

bacteria and yeast sample(s)

storage temp.

−20°C

Application

All the advantages of CelLytic™ Express now in a tablet format. For direct lysis of bacterial cultures and for use in the His-Select® iLAP™ column. CelLytic Express tablets are a complete formulation including lysozyme and endonuclease for complete cell lysis. The resulting lysate is clear of cellular debris, and is immediately ready for standard affinity purification. This new formulation adds minimal volume to the final lysate, providing a method with defined volume and time parameters, making it easier to validate and making it more convenient for production scale protocols.

Features and Benefits

  • Efficient and non-denaturing in-culture protein extraction
  • Save time by eliminating cell harvest and clarification steps
  • Preserve biological activity with fewer manipulations
  • Compatible with affinity resins and magnetic beads
  • Ideal for Production Scale Extraction protocols

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
iLAP is a trademark of Sigma-Aldrich Co. LLC

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This Item
C2978C4482524750
description

1 tablet sufficient for 1 mL lysis (cell suspension)

description

Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.

description

For yeast cells

description

Dissolving one tablet in 500 ml of deionized H2O yields 150 mM NaCl, 50 mM Tris-HCl buffer, pH 7.6 at 25°C. Supplied in convenient blister packs. Note: 1 each = 10 tablets.

usage

1 tablet sufficient for 1 mL lysis (cell suspension)

usage

-

usage

-

usage

-

form

tablet

form

solution

form

solution

form

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

100

storage temp.

−20°C

storage temp.

room temp

storage temp.

-

storage temp.

15-25°C

input

bacteria and yeast sample(s)

input

mammalian sample(s)

input

bacteria and yeast sample(s)

input

-


Pictograms

Exclamation mark

Signal Word

Warning

Hazard Codes

Precautionary Statements

Hazard Classifications

Acute Tox. 4 Oral

Storage Class

11 - Combustible Solids

WGK

WGK 3



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