C7401

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit

fractionated antiserum, lyophilized powder

• Synonym(s): Anti-Con A, Anti-Jack Bean Concanavalin A
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00162209
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ELISA (i)
• Citations: 7

Properties

• biological source: rabbit
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: fractionated antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: lyophilized powder
• species reactivity: Canavalia ensiformis
• packaging: vial of ~2 mL lyophilized antiserum
• technique(s): indirect ELISA: 1:25,000
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Lectins are highly specific carbohydrate binding proteins useful in carbohydrate and cell membrane studies, as well as cell agglutination and typing techniques.

Immunogen

Jack Bean Concanavalin A (Con A).

Application

Anti-Lectin, Canavalia ensiformis antibody produced in rabbit has been used in:

• enzyme-linked immunosorbent assays (ELISA)
• immunochemistry
• enhanced chemiluminescence ECL^™ western blot analysis
• western blot

Biochem/physiol Actions

Concanavalin A is a lectin isolated from Canavalia ensiformis or Jack bean seeds. It has potent mitogenic effect on T cells and triggers the activity of both helper and suppressor T cells exhibiting immune regulator activity. Con A triggers autophagy by binding on the cell membrane glycoprotein and is internalized to the mitochondria. It activates immune response in liver and displays anti-hepatoma effect.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.2.

Preparation Note

Prior to reconstitution store the product at 2-8 °C. After reconstitution, the solution may be stored frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage clarify the solution by centrifugation before use.

Reconstitute with 2 mL deionized water.

Legal Information

ECL is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)