C7974

Monoclonal Anti-Collagen, Type X antibody produced in mouse

clone COL-10, ascites fluid

• Synonym(s): Anti-Col10, Anti-Col10a-1
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD02686071
• Conjugate: unconjugated
• Clone: COL-10, monoclonal
• Application: DB, ICC, WB
• Citations: 42

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: COL-10, monoclonal
• mol wt: antigen 60 kDa (in denatured-reduced preparations)
• contains: 15 mM sodium azide
• species reactivity: deer, human, pig
• technique(s): dot blot: suitable, immunocytochemistry: 1:1,000 using HT 1080 human fibrosarcoma cells, western blot: suitable
• isotype: IgM
• UniProt accession no.: Q03692
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... COL10A1(1300)

Description

General description

Collagens are extracellular glycoproteins made up of three polypeptides that intermingle to form a triple helix. Type X collagen is a homotrimer of 59 kD α1(X) chains found in fetal hypertrophic cartilage in the growth zones of long bones, vertebrae and ribs, whereas in adults it is also present in thyroid cartilage. Monoclonal anti-collagen, type X antibody can be used in histological and immunohistochemical evaluation of cell cultures. It is also useful in study of specific differential tissue expression.

Monoclonal Anti-Collagen, Type X (mouse IgM isotype) is derived from the COL-10 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with porcine collagen type X. Collagen type X shares a similar domain structure with type VIII collagen: a central triple-helical (COL1) domain of 50 kDa is flanked by N-terminal (NC2) and C-terminal (NC1) non-triple-helical domains.

Immunogen

purified pig collagen type X.

Application

Monoclonal Anti-Collagen, Type X antibody produced in mouse has been used in

• enzyme linked immunosorbent assay (ELISA)
• dot-blot
• immunoblotting and
• immunohistochemistry

Biochem/physiol Actions

Type X collagen is a product of hypertrophic chondrocytes. Type X collagen is non-fibrillar, but forms fine pericellular filaments in association with cartilage collagen. It interacts with matrix proteins, such as connexin V, chondrocalcein, collagen II and proteoglycans, as well as with Ca²⁺. It acts as a scaffold to avoid local collapse during endochondral ossification and also has a role in cartilage mineralization. Mutations in the NC1-encoding domain of the human α1(X) collagen gene, are associated with Schmid metaphysical chondroplasia.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable