C8740
CelLytic™ B Cell Lysis Reagent
For bacterial cell lysis, 10× concentrate
Synonym(s):
bacterial cell lysis reagent
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| Size/SKU | Availability | Price |
|---|---|---|
10 mL | Please contact Customer Service for Availability | 2 970,00 CZK |
50 mL | Please contact Customer Service for Availability | 9 070,00 CZK |
100 mL | Please contact Customer Service for Availability | 15 500,00 CZK |
About This Item
2 970,00 CZK
Please contact Customer Service for Availability
Application
A proprietary, non-denaturing formulation of zwitterionic detergents used for the lysis of bacterial cells and extraction of recombinant proteins.
TM[="CelLytic"] B Cell Lysis Reagent has been used for lysing E.coli 5-helix bundle protein. It is also suitable for lysing hepatitis B X-interacting protein (HBXIP) and E. coli TG1 cells.
Features and Benefits
- Higher protein extraction efficiency than traditional methods such as sonication and lysozyme
- Scalable for 1 to 25 grams of bacterial cell paste
- No interference with downstream applications such as affinity chromatography, IP, and Western blotting
- Compatible with protease inhibitors, inhibitor cocktails, chaotropes, salts, chelating agents and reducing agents
Other Notes
10X concentrate of detergent blend contains no buffering components, giving the researcher flexibility to integrate the lysis reagent into their desired system. Also amenable to automation protocols for recombinant protein extraction and purification.
Legal Information
Covered by US Patent No 7,282,475 B2 and are sold for research use only. Commercial use requires addtional licenses.
CelLytic is a trademark of Sigma-Aldrich Co. LLC
1 of 1
This Item | |||
|---|---|---|---|
| description For bacterial cell lysis, 10× concentrate | description For yeast cells | description solution, Roche, pkg of 100 mL, sufficient for 50-500 reactions | description sufficient for 100 reactions, Molecular Biology |
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 200 |
| form liquid | form solution | form solution | form liquid |
| input bacteria and yeast sample(s) | input bacteria and yeast sample(s) | input - | input - |
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Signal Word
Warning
Hazard Codes
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
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