C9282

Sodium cholate hydrate

suitable for cell culture, BioReagent

• Synonym(s): 3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid sodium salt, Cholalic acid sodium salt
• Empirical Formula (Hill Notation): C₂₄H₃₉NaO₅ · xH₂O
• CAS Number: 206986-87-0
• Molecular Weight: 430.55 (anhydrous basis)
• UNSPSC Code: 12161902
• NACRES: NA.75
• PubChem Substance ID: 24893147
• EC Number: 206-643-5
• Beilstein/REAXYS Number: 3582354
• MDL number: MFCD00150749

Properties

• Quality Segment: 200
• biological source: bovine bile, ovine bile
• description: anionic
• product line: BioReagent
• assay: ≥99.0% (HPLC)
• form: powder
• mol wt: micellar avg mol wt 900-1300
• aggregation number: 2-3
• technique(s): cell culture | mammalian: suitable
• CMC: 9-15 mM (20-25°C)
• solubility: water: 100 mg/mL, clear, colorless to light yellow
• HLB: 18
• SMILES string: O.[Na+].C[C@H](CCC([O-])=O)C1CCC2C3[C@H](O)CC4C[C@H](O)CC[C@]4(C)C3C[C@H](O)[C@]12C
• InChI: 1S/C24H40O5.Na.H2O/c1-13(4-7-21(28)29)16-5-6-17-22-18(12-20(27)24(16,17)3)23(2)9-8-15(25)10-14(23)11-19(22)26;;/h13-20,22,25-27H,4-12H2,1-3H3,(H,28,29);;1H2/q;+1;/p-1/t13-,14+,15-,16-,17+,18+,19-,20+,22+,23+,24-;;/m1../s1
• InChI key: MUVVIYFKOVLQHL-RCVKHMDESA-M

Description

General description

Cholate is a primary bile acid produced from cholesterol in the liver. It is an anionic detergent synthesized from cholesterol in the presence of sterol 12α-hydroxylase (CYP8B1).

Application

Sodium cholate hydrate has been used:

• as a bile acid to induce bile acid-responsive (BEAR) transcription system,
• as a component of Campylobacter defined broth (CDB) to test its effect on stress response in Campylobacter jejuni{52
• to test its sensitivity towards the bile acid sensor

Cholate is a bile acid produced in liver during cholesterol breakdown. Cholate downregulates cholesterol-7-α-hydroxylase (rate-limiting step in bile acid synthesis). Cholate, an anionic detergent, is used alone and in combination with urea to extract and reconstitute membrane proteins and protein complexes.

Biochem/physiol Actions

Reduction in 12-α hydroxylase activity leads to low levels of cholic acid in liver disease.

Safety Information

• Hazard Codes: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)