CHP-134

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 6122002, human nerve (neuroblastoma), Neuronal

• Synonym(s): CHP134 Cells, Children′s Hospital of Philadelphia-134 Cells
• UNSPSC Code: 41106514
• Biological source: human nerve (neuroblastoma)
• Relevant disease(s): metastasis
• Growth mode: Adherent
• Karyotype: Not specified
• Morphology: Neuronal
• Manufacturer/tradename: ECACC

Properties

• biological source: human nerve (neuroblastoma)
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 06122002-DNA-5UG, pkg of vial of cells 06122002-1VL
• growth mode: Adherent
• karyotype: Not specified
• morphology: Neuronal
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): metastasis
• shipped in: dry ice
• storage temp.: −196°C

Description

Biochem/physiol Actions

A neuroblastoma cell line established from a tumour of the left adrenal area of an 18-month-old male patient. The patient was previously treated with vincristine, cyclophosphamide, dimethyltriazenoimidazole carboxamide, adriamycin, and irradiation. The cells grow as adherent colonies consisting of neuroblast-like cells forming dense mounds. Individual cells radiate from the mounds which reach confluency after routine passaging for several months. This cell line has been reported to have tyrosine hydroxylase activity.

Human neuroblastoma tumour mass of left adrenal gland

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 8,11
D16S539: 11,13
D5S818: 11,12
D7S820: 9
THO1: 9,9.3
TPOX: 8
vWA: 18,19

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split confluent cultures 1:2 to 1:10 every 2-3 days, use gentle agitation to dislodge cells, trypsin/EDTA should not be necessary as cells only adhere loosely; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.