OE21 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96062201, human esophagus, Epithelial

• Synonym(s): JROECL 21 Cells, OE 21 Cells
• UNSPSC Code: 41106514
• Biological source: human esophagus
• Relevant disease(s): cancer
• Growth mode: Adherent
• Karyotype: Aneuploid
• Morphology: Epithelial
• Manufacturer/tradename: ECACC

Properties

• biological source: human esophagus
• manufacturer/tradename: ECACC
• packaging: tube of 5 μg 96062201-DNA-5UG, pkg of vial of cells 96062201-1VL
• growth mode: Adherent
• karyotype: Aneuploid
• morphology: Epithelial
• products: Not specified
• receptors: MHC class I
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Application

Study of oesophageal cancer

Biochem/physiol Actions

Human Caucasian oesophageal squamous cell carcinoma

OE21, also known as JROECL21, was established in 1993 from a squamous carcinoma of mid oesophagus of a 74 year-old male patient. The tumour was identified as pathological stage IIA(UICC) and showed moderate differentiation. HLA-A,-B and -C (MHC class I) are expressed constitutively, expression of ICAM-1 can be induced by treatment with interferon-gamma. OE21 cells express epithelial cytokeratins and are tumourigenic in nude mice.

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 12
D16S539: 13
D5S818: 12,13
D7S820: 11,12
THO1: 8
TPOX: 8,11
vWA: 16,17

Preparation Note

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:8 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.