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CS0170

Thioredoxin Reductase Assay Kit

1 kit sufficient for 100 assays (1 mL)

Synonym(s):

Thioredoxin Reductase Activity Kit

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1 kit
Please contact Customer Service for Availability
18 800,00 CZK
15 416,00 CZK

About This Item

NACRES:
NA.84
UNSPSC Code:
12161503

15 416,00 CZK

List Price18 800,00 CZKSave 18%

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Quality Segment

usage

 kit sufficient for 100 assays (1 mL)

shipped in

dry ice

storage temp.

−20°C

Gene Information

General description

The Thioredoxin Reductase Assay Kit uses a colorimetric assay for the determination of thioredoxin reductase activity in mammalian cells and tissues. It is based on the reduction of 5,5′-dithiobis(2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) that produces a strong yellow color that is measured at 412 nm.

DTNB + NADPH + H+ ↔ 2 TNB + NADP+

Application

Using post nuclear extracts of rat liver, the kit shows linearity up to 0.25 units per mL. In this preparation, ~20% of the activity is due to non-specific reductase activity (refractory to the TrxR inhibitor).

Features and Benefits

  • An easy and simple method
  • The kit contains all the reagents required for a colorimetric assay of mammalian thioredoxin reductase
  • The kit includes an inhibitor solution for specific inhibition of mammalian thioredoxin reductase
  • The kit has been tested with heart muscle, as well as lysates from cell lines such as HeLa, A549, Jurkat, U937, A431, COS, CHO, and NIH 3T3 cells

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This Item
T9698GRSACS0340
description

1 kit sufficient for 100 assays (1 mL)

description

buffered aqueous glycerol solution, ≥100 units/mg protein (Bradford)

description

Sufficient for 100 colorimetric tests

description

1 kit sufficient for 50-100 tests

Gene Information

human ... TXNRD1(7296), TXNRD2(10587), TXNRD3(114112)

Gene Information

rat ... Txnrd1(58819)

Gene Information

human ... GSR(2936)

Gene Information

human ... DHFR(1719)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

 kit sufficient for 100 assays (1 mL)

usage

-

usage

sufficient for 100 tests

usage

 kit sufficient for 50-100 tests

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

shipped in

dry ice

shipped in

dry ice

shipped in

-

shipped in

dry ice


Kit Components Only

Product No.
Description

  • Assay Buffer 5x for Thioredoxin Reductase 30 mL

  • Thioredoxin Reductase 10 μg

  • Thioredoxin Reductase Inhibitor Solution .05 mL

  • 5,5'-Dithiobis(2-nitrobenzoic) acid (DNTB) 150 mg

  • NADPH 25 mg

  • Dimethyl Sulfoxide (DMSO) 7.5 mL

Signal Word

Warning

Storage Class

10 - Combustible liquids

Flash Point (°F)

188.6 °F

Flash Point (°C)

87 °C

Pictograms

Health hazardExclamation mark

Hazard Codes

Hazard Classifications

Eye Irrit. 2 - Repr. 2 - STOT SE 2

Target Organs

Eyes,Central nervous system

WGK

WGK 3



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