D2001

Deoxyribonucleic acid sodium salt from Escherichia coli strain B

Type VIII

• Synonym(s): DNA
• CAS Number: 93384-16-8
• UNSPSC Code: 41106305
• eCl@ss: 32160414
• EC Number: 297-226-7
• NACRES: NA.51
• MDL number: MFCD00130922
• Form: fibers
• Solubility: water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
• Storage temp.: 2-8°C

Properties

• type: Type VIII
• Quality Segment: 200
• form: fibers
• impurities: <2% protein
• color: , white to white with yellow cast
• solubility: water: 0.98-1.02 mg/mL, faintly hazy to hazy, colorless to light yellow
• λmax: 256-264 nm (15mM NaCl+1.5mM Trisodium Citrate PH7)
• storage temp.: 2-8°C

Description

Application

Escherichia coli DNA has many uses in research. Escherichia coli DNA (ecDNA) is used in studies of DNA binding agents that modulate DNA structure and function. E. coli DNA is used in physicochemical studies of DNA behavior in solution, and to evaluate species-specific DNA structures and binding domains. This DNA is particularly useful for genomic analysis, including PCR, library construction in bacteriophage lambda, and in heterologous pre-hybridization and hybridization protocols.

Deoxyribonucleic acid sodium salt from Escherichia coli strain B has been used to measure the melting temperature (Tm) and in differential scanning calorimetry (DSC) studies. It has also been used as a template sequence in the polymerase chain reaction (PCR).

Preparation Note

Prepared by procedure of Marmur, J., J. Mol. Biol., 3, 208 (1961).

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)