D2191

Monoclonal Anti-DC-SIGN1 antibody produced in mouse

clone 120507, purified immunoglobulin, lyophilized powder

• Synonym(s): Anti-CD209, Anti-Dendritic Cell-Specific ICAM-3 Grabbing Non-Integrin
• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD03701938
• Conjugate: unconjugated
• Clone: 120507, monoclonal
• Application: FACS, ICC, IHC, WB
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 120507, monoclonal
• form: lyophilized powder
• species reactivity: human
• technique(s): flow cytometry: 2.5 μg using 10⁶ cells, immunocytochemistry: 8-25 μg/mL, immunohistochemistry: 8-25 μg/mL using fixed cells and tissue sections, western blot: 1 μg/mL
• isotype: IgG2b
• UniProt accession no.: Q9NNX6
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CD209(30835)

Description

General description

DC-SIGN is a type II protein C-type lectin that binds mannose-rich molecules such as ICAM-3 and ICAM-2 in a calcium dependent manner. DC-SIGN, a 44 kDa molecule, is identical to the HIV-1 binding protein.

Immunogen

purified from NIH/3T3 cells transfected with recombinant human DC-SIGN.

Application

Monoclonal Anti-DC-SIGN1 antibody produced in mouse is suitable for flow cytometry, immunocytochemistry at a working concentration of 8-25 μg/mL, immunohistochemistry at 8-25 μg/mL using fixed cells and tissue sections and western blotting at 1 μg/mL.

Biochem/physiol Actions

DC-SIGN1 is involved in molecular interactions between dendritic cells and resting T-cells. DC-SIGN interacts with viruses such as X4, R5, X4R5 HIV-1 strains, HIV-2, and SIV. It captures HIV-1 through its interaction with the gp120 envelope glycoprotein and promotes efficient infection of CD4⁺ T cells that co-express chemokine receptors.

Recognizes human DC-SIGN on transfected cells and on monocyte derived dentritic cells. The antibody does not react with either parental mouse cells or irrelevant transfectants, such as human DC-SIGN2.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)