We only use this product for our primary cell and cell line cultures.
D5648
DMEM - high glucose
With ʟ-glutamine, without sodium bicarbonate and sodium pyruvate, powder, suitable for cell culture
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM
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| Size/SKU | Availability | Price |
|---|---|---|
10 L | Please contact Customer Service for Availability | 1 250,00 CZK |
10 x 1 L | Please contact Customer Service for Availability | 1 910,00 CZK |
50 L | Please contact Customer Service for Availability | 4 570,00 CZK |
About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture
Quality Segment
form
powder
technique(s)
cell culture | mammalian: suitable
components
phenol red: yes
L-glutamine: yes
NaHCO3: no
HEPES: no
glucose: high
sodium pyruvate: no
shipped in
ambient
storage temp.
2-8°C
General description
Application
Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".
Dulbecco′s Modified Eagle′s Medium - high glucose has been used as the culture medium for various cell types including the human cervical endocervical epithelial End1/E6E7 cells, human adenocarcinoma epithelial cells (HeLa), human alveolar adenocarcinoma epithelial cells (A549), human osteosarcoma epithelial cells, human diploid cell line , primary hepatocytes and synovial tissue
Preparation Note
Formulated to contain 13.4 grams of powder per liter of medium.
Supplement with 3.7 g/L sodium bicarbonate.
1 of 1
This Item | |||
|---|---|---|---|
| description With 4500 mg/L glucose and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture | description With 4500 mg/L glucose, L-glutamine, and sodium pyruvate, without sodium bicarbonate, powder, suitable for cell culture | description With 4500 mg/L glucose, L-glutamine, sodium pyruvate, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture | description DMEM/High Modified, with 4500 mg/L dextrose, with 110 mg/L sodium pyruvate, without ferric nitrate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture |
| form powder | form powder | form liquid | form liquid |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| components phenol red: yes | components glucose: high | components HEPES: no | components L-glutamine: no |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 500 | Quality Level 500 | Quality Level 400 | Quality Level 500 |
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Storage Class
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
10043-52-4
CAS No.
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