The concentration of the DNA marker is not part of the specification and that information is not available. Product DPLUS-1K - DirectLoad™ Plus 1kb DNA Ladder is a comparable marker that may be considered an alternative. DPLUS-1KT is noted as having a DNA concentration of 100 ug/ul. The DNA concentration for individual bands is unavailable.
D7058
DirectLoad™ Wide Range DNA Marker
ready-to-use marker for DNA electrophoresis
Synonym(s):
agarose gel electrophoresis marker, gel ladder, gel marker, wide range ladder
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1 VIAL
CZK 9,920.00
CZK 9,920.00
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About This Item
UNSPSC Code:
41105335
NACRES:
NA.25
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Quality Level
form
liquid
usage
100 uses
solubility
water: soluble
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
1 of 4
This Item | D3937 | D3687 | P9577 |
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form liquid | form liquid | form liquid | form liquid |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| usage 100 uses | usage 100 uses | usage 75 uses | usage sufficient for 50 uses |
| suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) | suitability suitable for electrophoresis (DNA) |
NA electrophoresis | NA electrophoresis, ladder, marker | - | - |
General description
Sigma′s DirectLoad Wide Range DNA Marker contains 16 fragments from 50 – 10,000 bp, with bands regularly spaced. Ten ul of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.
Features and Benefits
- Ready-to-load
- Easy-to-use
- Popular band sizes
Other Notes
Contains 16 fragments, 50–10,000 bp which produce logically spaced bands on an agarose gel.
For optimal resolution, the recommended agarose gel concentration is 0.75 – 2.0%.
Sigma′s DirectLoad™ Wide Range DNA Marker is provided in a gel-loading solution of 2.5% Ficoll (Type 400), 0.0125% bromophenol blue, and 0.00625% xylene cyanol.
Legal Information
DirectLoad is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Melanie P Dautle et al.
Journal of clinical microbiology, 40(2), 414-421 (2002-02-05)
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Datasheet
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Could you provide the concentraion of the marker? Idealy band by band so that it can be used as an estimate of the concentration of samples run along side.
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