E0778

Ephrin-B2/Fc Chimera from mouse

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• Synonym(s): ELF-2, Htk-L, LERK-5, NLERK-1
• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD01867533

Properties

• biological source: mouse
• Quality Segment: 200
• recombinant: expressed in NSO cells
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• mol wt: monomer calculated mol wt 49.6 kDa, 60-65 kDa by SDS-PAGE (reducing)
• packaging: pkg of 200 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• UniProt accession no.: P52800
• storage temp.: −20°C
• Gene Information: mouse ... Efnb2(13642)

Description

Biochem/physiol Actions

Member of ephrin ligand family that binds Eph receptor tyrosine kinases (EphA4, EphB1, EphB2, EphB3, and EphB4). Involved in pattern formation and morphogenesis. Ephrin-B2 can be detected on monocytes, mesangial cells, CD34⁺ stem cells, bone marrow fibroblasts, activated melanocytes and melanoma cells, and pre-artery endothelial cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline.

Analysis Note

The biological activity is measured by its ability to bind immobilized recombinant mouse EphB2/Fc in ELISA.

Other Notes

Extracellular domain of mouse ephrin-B2 (amino acids 1-227) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, type N95 (US)