E1235

EGF human

Xeno-free, recombinant, expressed in HEK 293 cells, suitable for cell culture

• UNSPSC Code: 12352202
• NACRES: NA.77
• Form: lyophilized powder
• Mol wt: 7.3 kDa

Properties

• Quality Segment: 300
• form: lyophilized powder
• mol wt: 7.3 kDa
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: -10 to -25°C

Description

General description

Recombinant human Epidermal Growth Factor is small polypeptide of 6.2 kDa protein containing 53 amino acid residues. The EGF gene encodes for a large type I membrane–anchored precursor protein which is cleaved into pro-EGF for generation of peptide hormone EGF. EGF can be found in human platelets, macrophages, urine, saliva, milk, and plasma.

Biochem/physiol Actions

Epidermal Growth Factor (EGF) is a growth factor that plays an important role in the regulation of cell growth, proliferation, and differentiation by binding to its receptor EGFR. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

Preparation Note

For reconstitution: Briefly centrifuge the vial before opening. Reconstitute in water to a concentration of 0.1 mg/mL. Do not vortex. This solution is recommended to store in working aliquots at –20 oC to -80 oC. Store the lyophilized product at –20 oC. The product is stable for at least 2 years as supplied.

Safety Information

• Storage Class: 11 - Combustible Solids
• WGK: WGK 2